6S RNA jako nástroj pro navýšení produkce antibiotik u Streptomyces coelicolor

Bc. Inesa MUSIĆ

Master's thesis

6S RNA as a Tool to Increase Production of Antibiotics in Streptomyces coelicolor

Abstract:

This diploma thesis focuses on the phenotypic effect of 6S RNA in model organism Streptomyces coelicolor. 6S RNA is small non-protein-coding RNA. As it was shown in Escherichia coli, 6S RNA imitates structure of DNA promoter and traps sigma70-RNA polymerase, consequently blocking further transcription. Recently, 6S-like RNA was also discovered in Streptomyces (designated here as 6SSc for clarity), but its effect on their complex life cycle remains unknown. Here, we tested whether 6SSc co-regulates transition from the vegetative growth to the stationary phase. When 6SSc RNA was overexpressed in S. coelicolor, this transition was reflected by limited substrate mycelium growth hyper-sporulation, and an over-production of secondary metabolites, actinorhodin and undecylprodigiosin, whose production was measured spectrophotometrically. Hence, we hypothesize that this RNA regulator may also affect the production of other bioactive substances in Streptomyces, including clinically relevant antibiotics.

Abstract:

This diploma thesis focuses on the phenotypic effect of 6S RNA in model organism Streptomyces coelicolor. 6S RNA is small non-protein-coding RNA. As it was shown in Escherichia coli, 6S RNA imitates structure of DNA promoter and traps sigma70-RNA polymerase, consequently blocking further transcription. Recently, 6S-like RNA was also discovered in Streptomyces (designated here as 6SSc for clarity), but its effect on their complex life cycle remains unknown. Here, we tested whether 6SSc co-regulates transition from the vegetative growth to the stationary phase. When 6SSc RNA was overexpressed in S. coelicolor, this transition was reflected by limited substrate mycelium growth hyper-sporulation, and an over-production of secondary metabolites, actinorhodin and undecylprodigiosin, whose production was measured spectrophotometrically. Hence, we hypothesize that this RNA regulator may also affect the production of other bioactive substances in Streptomyces, including clinically relevant antibiotics.

Keywords

Streptomyces coelicolor, 6S RNA, actinorhodin, undecylprodigiosin, secondary metabolism

Language used: English

Date on which the thesis was submitted / produced: 23. 4. 2018

Thesis defence

• Supervisor: RNDr. Jan Bobek, Ph.D.

JAN EVANGELISTA PURKYNĚ UNIVERSITY IN ÚSTÍ NAD LABEM

Faculty of Science

Master programme / field:
Biology / Biology