Genetic engineering of psbA gene in Nicotiana tabacum – Bc. Dagmar HUCKOVÁ
Bc. Dagmar HUCKOVÁ
Master's thesis
Genetic engineering of psbA gene in Nicotiana tabacum
Abstract:
Transformation vector with mutated psbA gene and selective aadA gene was created and transferred into Nicotiana tabacum living cells using Biolistic bombardment. Due to homologous recombination, transformed plant lineage carrying D1-A209, D1-C-212 instead of D1-S209, D1-S212 in D1 protein in PS II was obtained. Seeds from transformed plant were harvested and homoplasmy of the first generation was tested …moreAbstract:
Transformation vector with mutated psbA gene and selective aadA gene was created and transferred into Nicotiana tabacum living cells using Biolistic bombardment. Due to homologous recombination, transformed plant lineage carrying D1-A209, D1-C-212 instead of D1-S209, D1-S212 in D1 protein in PS II was obtained. Seeds from transformed plant were harvested and homoplasmy of the first generation was tested …more
Language used: English
Date on which the thesis was submitted / produced: 13. 12. 2017
Thesis defence
- Supervisor: Mgr. David Kaftan, Ph.D.
Citation record
ISO 690-compliant citation record:
HUCKOVÁ, Dagmar. \textit{Genetic engineering of psbA gene in Nicotiana tabacum}. Online. Master's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2017. Available from: https://theses.cz/id/q0qrir/.
The right form of listing the thesis as a source quoted
HUCKOVÁ, Dagmar. Genetic engineering of psbA gene in Nicotiana tabacum. Č. Budějovice, 2017. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceMaster programme / field:
Biology / Experimental Biology
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