Detection of intracellular H2O2 molecules using the genetically encoded fluorescent marker HyPer7 – Bc. Miroslava BROMOVÁ
Bc. Miroslava BROMOVÁ
Master's thesis
Detection of intracellular H2O2 molecules using the genetically encoded fluorescent marker HyPer7
Abstract:
In order to discern the role that mitochondrial reactive oxygen species (ROS) play during procyclic Trypanosoma brucei differentiation, we implemented the next-generation hydrogen peroxide biosensor Hyper7 to detect when and where ROS is being generated. Hyper7 fluorescence assays were optimized to determine the efficacy of this probe in an evolutionary divergent organism. In addition, we initiated …moreAbstract:
In order to discern the role that mitochondrial reactive oxygen species (ROS) play during procyclic Trypanosoma brucei differentiation, we implemented the next-generation hydrogen peroxide biosensor Hyper7 to detect when and where ROS is being generated. Hyper7 fluorescence assays were optimized to determine the efficacy of this probe in an evolutionary divergent organism. In addition, we initiated …more
Language used: English
Date on which the thesis was submitted / produced: 12. 4. 2024
Thesis defence
- Supervisor: doc. RNDr. Alena Panicucci Zíková, Ph.D.
Citation record
ISO 690-compliant citation record:
BROMOVÁ, Miroslava. \textit{Detection of intracellular H2O2 molecules using the genetically encoded fluorescent marker HyPer7}. Online. Master's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2024. Available from: https://theses.cz/id/qi6xgm/.
The right form of listing the thesis as a source quoted
BROMOVÁ, Miroslava. Detection of intracellular H2O2 molecules using the genetically encoded fluorescent marker HyPer7. České Budějovice, 2024. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceMaster programme / field:
Chemistry / Biochemistry
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