Analýza hořkých kyselin v pivu s využitím disperzní kapalinové mikroextrakce

Bc. Monika Kadrmasová

Master's thesis

Analysis of beer bitter acids by means of dispersive liquid-liquid microextraction

Abstract:

Tato diplomova prace je zam..ena na vyu.iti disperzni kapalinove mikroextrakce ve spojeni s kapalinovou chromatografii s UV detekci pro stanoveni ?ż- a ?Ŕ-ho.kych kyselin v klasickem pivu a v pivu chmelenem za studena. V teoreticke .asti jsou popsany r.zne zp.soby modifikace disperzni kapalinove mikroextrakce, mo.nosti spojeni teto mikroextrakce s kapalinovou chromatografii a dal.imi technikami. Zam..ena je i na vyrobu piva, popis r.znych zp.sob. chmeleni a stanoveni latek obsa.enych v pivu, zejmena t.kavych latek a ho.kych kyselin. V experimentalni .asti byly nejprve optimalizovany podminky 4 disperznich kapalinovych mikroextrakci vyu.ivajici ztuhnuti organicke kapky (DLLME-SFO, AA-LLME-SFO, UA-LLME-SFO, UA-DLLME-SFO), ze kterych byla vybrana nejvhodn.j.i mikroextrakce pro stanoveni ho.kych kyselin. Dale jsou prezentovany vysledky analyzy realnych vzork. piv a porovnani mno.stvi ho.kych kyselin v pivech chmelenych klasicky a pivech chmelenych za studena.

Abstract:

This thesis deals with the use of dispersive liquid-liquid microextraction followed by liquid chromatography with UV detection for the determination of á- and â-bitter acids in classical beer and in dry-hopped beer. In the theoretical part there are described various modifications of dispersive liquid-liquid microextraction methods together with possibilities of their connection with liquid chromatography and other techniques. It is also focused on the production of beer, the description of the different ways of hoping and the determination of substances in beer, especially volatile compounds and bitter acids. In the experimental part, the conditions of the 4 dispersive liquid-liquid microextraction methods with solidification of floating organic drop (DLLME-SFO, AA-LLME-SFO, UA-LLME-SFO, UA-DLLME-SFO) were optimised and the most suitable approach for the determination of bitter acids was selected. In addition, the results of the analysis of real beer samples and the comparison of bitter acids in classical beer and dry-hopped beer are presented.

Keywords

Disperzní kapalinová mikroextrakce, kapalinová chromatografie, pivo, studené chmelení, hořké kyseliny

Language used: Czech

Date on which the thesis was submitted / produced: 11. 5. 2018

Accessible from:: 31. 12. 2999

Thesis defence

• Supervisor: doc. Ing. Martin Adam, Ph.D.

University of Pardubice

Faculty of Chemical Technology

Master programme / field:
Chemistry and Technology of Foodstuffs / Evaluation and Analysis of Foodstuffs