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Mgr. Roman Sándor

Bachelor's thesis

Heterologní exprese cryptogeinu K39T/K94T v systému kvasinky Pichia pastoris

Heterologous expression of K39T/K94T cryptogein in Pichia pastoris system
Abstract:
Pichia pastoris je eukaryotická kvasinka, schopná post translačních úprav proteinů, což je velká výhoda oproti běžně v biotechnologiích využívané E. coli. Táto kvasinka je schopna metabolizovat metanol a její gen zodpovědný za expresi alkoholoxydázy (AOX1) je zároveň silný inducibilný promotor. Protein, který byl v naší práci exprimován je cryptogein, protein z rodiny elicitinů, produkovaných patogenními …more
Abstract:
Pichia pastoris is an eukaryotic yeast, able to do posttranslational modifications of proteins and protein folding in comparison to other microorganism used in biotechnologies, E. coli. This yeast is also able to metabolize methanol and the gene responsible for expression of alcoholoxidase responsible (AOX1) is utilized as strong promoter. The protein expressed in our work is called cryptogein. Cryptogein …more
 

Keywords

Pichia pastoris Proteiny Purifikace proteinú Kryptogein Fermentor Kvasinka Purification of proteins Fermenter Cryptogein Yeast
 
Language used: English
Date on which the thesis was submitted / produced: 14. 5. 2012
Identifier: https://is.muni.cz/th/jcr11/

Thesis defence

  • Date of defence: 19. 6. 2012
  • Supervisor: doc. Mgr. Jan Lochman, Ph.D.

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Institution archiving the thesis and making it accessible: Masarykova univerzita, Přírodovědecká fakulta
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