Mechanisms of dsRNA virus replication: Cloning, production and structural characterization of C-terminal domain of sigmaNS. – Nelli Vanessza MANOCZKI
Nelli Vanessza MANOCZKI
Bachelor's thesis
Mechanisms of dsRNA virus replication: Cloning, production and structural characterization of C-terminal domain of sigmaNS.
Mechanisms of dsRNA Virus Replication: Cloning, Production and Structural Characterization of C-Terminal Domain of ZNS.
Abstract:
A gene fragment encoding the C-terminal domain of the sigmaNS protein of the avian reovirus was amplified via PCR. The amplicon was cloned using BsaI restriction enzyme into the pASKIBA37+ expression vector and transformed into One ShotTM TOP 10 Chemically Competent E.coli cells. Colony PCR was performed and plasmids were sent for sequencing. Sequence verified plasmids were transformed into One ShotTM …moreAbstract:
A gene fragment encoding the C-terminal domain of the sigmaNS protein of the avian reovirus was amplified via PCR. The amplicon was cloned using BsaI restriction enzyme into the pASKIBA37+ expression vector and transformed into One ShotTM TOP 10 Chemically Competent E.coli cells. Colony PCR was performed and plasmids were sent for sequencing. Sequence verified plasmids were transformed into One ShotTM …more
Language used: English
Date on which the thesis was submitted / produced: 14. 5. 2019
Thesis defence
- Supervisor: RNDr. Zdeněk Franta, Ph.D.
Citation record
ISO 690-compliant citation record:
MANOCZKI, Nelli Vanessza. \textit{Mechanisms of dsRNA virus replication: Cloning, production and structural characterization of C-terminal domain of sigmaNS.}. Online. Bachelor's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2019. Available from: https://theses.cz/id/rc3fzz/.
The right form of listing the thesis as a source quoted
MANOCZKI, Nelli Vanessza. Mechanisms of dsRNA virus replication: Cloning, production and structural characterization of C-terminal domain of sigmaNS.. Č. Budějovice, 2019. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceBachelor programme / field:
Biochemistry / Biological Chemistry
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