Molecular Mechanism of Apoptosis in Neutrophil Granulocytes Compared to Septic Granulocytes

MUDr. Ladislav Mica, Ph.D.

Doctoral thesis

Abstract:

Regulace bunecne smrti Apoptozou je velmi dulezity proces u vysoce organizovanych organizmu na zachovani homeostazy a zabraneni bunecne desorganzace jak je tumorigeneza. V tomto pripade byla studovana Apoptoza neutrofilnich granulocytu (PMN). Polytraumatizovani pacienti velmi casto vyviji septicke komplikace kde je phyziologicka PMN Apoptoza zignifikantne redukovana. Povazujic redukci PMN apoptozi behem sepse jako patognomonikum, tak by porozumneni molekularnich mechanizmu v teto situaci mohle zlepsit phamakoterapii behem sepse. Jenom male okenko zde bylo otevreno na mozny mechanizmus apoptozy u PMN, dalsi prace musi overit zdali tento model odpovida realite.

Abstract:

Apoptotic regulation in highly organised organisms is extremely important to assure cellular homeostasis and to avoid cellular disorganisation, such as tumorigenesis. In this case the molecular mechanisms of apoptotic regulation were studied in neutrophil granulocytes (PMN). Polytrauma patients often develop septic complications where the natural apoptotic rate of PMN is significantly reduced. Understanding the molecular mechanisms of the anti-apoptotic regulation during sepsis could improve the pharmaco-therapeutic approach to sepsis. Here, we opened just a tiny window to a possible network of this signalling cascade. Future work has to prove if the shown model corresponds to the reality.

Keywords

Bc12, cIAP2, Proteasome, Apoptosis, PMN, SIRS, Sepsis, Trauma

Language used: Czech

Date on which the thesis was submitted / produced: 1. 12. 2010

Thesis defence

• Date of defence: 25. 10. 2011
• Supervisor: prof. MUDr. Jindřich Vomela, CSc.

Masaryk University

Faculty of Medicine

Doctoral programme / field:
Surgery (4-years) / Surgery