Instrumentální aspekty elektroforetické separace \nl{}v mikročipovém formátu

Bc. Martin Heinz

Bachelor's thesis

Instrumental Aspects of Electrophoretic Separations in Microchip Format

Abstract:

Cilem teto bakala.ske prace je vyvoj instrumentace pro mikro.ipovou elektroforezu zam..enou na separaci a detekci fluorescen.n. zna.enych oligosacharid. pomoci laserem indukovane fluorescence. Separa.ni kanaly o rozm.rech 25 x 60 ?Ęm a delce 65 mm byly vytvo.eny v borosilikatovem skle technikou fotolitografie a mokreho leptani. Propojeni mikro.ipu se zasobniky zakladnich elektrolyt., mikroskopu a vysokeho nap.ti bylo realizovano technologii 3D tisku z kyseliny polymle.ne. Mikrofluidni system byl charakterizovan pomoci zakladnich elektrolyt. kyseliny mraven.i, kyseliny octove a pomoci barviva methylenove mod.i. Dale byl vytvo.en, take technologii 3D tisku, fluorescen.ni detek.ni system.

Abstract:

The aim of this work is the development of instrumentation for microchip electrophoresis, focused on the separation and detection of fluorescently labeled oligosaccharides using laser induced fluorescence. The separation channels with dimensions of 25 x 60 microns and a length of 65 mm were formed in borosilicate glass photolithography technique and wet etching. Linking microchip bins essential electrolytes, microscope and high voltage was realized by 3D printing technology from polylactic acid. Microfluidic system was characterized by essential electrolytes formic acid, acetic acid and with methylene blue. Fluorescence detector was also created by 3D printing technology.

Keywords

Mikročipová elektroforéza, 3D tisk, fluorescenční detekce

Language used: Czech

Date on which the thesis was submitted / produced: 16. 7. 2015

Thesis defence

• Date of defence: 24. 8. 2015
• Supervisor: doc. Ing. Petr Česla, Ph.D.

University of Pardubice

Faculty of Chemical Technology

Bachelor programme / field:
Chemistry and Technical Chemistry / Chemistry and Technical Chemistry