Studium střevních prvoků rodu \kur{Blastocystis} v mikrobiomu člověka

Bc. Zuzana LHOTSKÁ

Master's thesis

Abstract:

The main aim of this study is to determine the prevalence and subtype diversity of the gut protist Blastocystis spp. in a volunteer group across the Czech Republic. Interestingly, Blastocystis has been recently considered as a part of the healthy gut microbiome in human. Beside the prevalence study, some factors such as animal contact, lifestyle, gender, and age have been associated with the presence of this gut protist in this sample dataset. We used two different diagnostic methods for detection of Blastocystis presence or absence: i) xenic cultivation and ii) specific PCR, based on which is also possible to distinguish Blastocystis subtypes. In total, we collected 411 samples from which 360 were processed within this study so far. Of these, the total prevalence of Blastocystis was determined - 21 % in humans and 9 % in animals. Nine subtypes were detected (ST1-ST7, ST10, ST14), of which six were detected in humans (ST1-ST4, ST6, ST7). We found out that some factors such as age, lifestyle (traveling, urban vs. rural) and contact with animals might affect the human colonization with Blastocystis, while we didn't detect any correlations in case of gender. Based on our results, we realized that PCR is more sensitive than cultivation

Jazyk práce: Czech

Datum vytvoření / odevzdání či podání práce: 16. 4. 2019

Obhajoba závěrečné práce

• Vedúci: MVDr. Kateřina Jirků-Pomajbíková, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Master programme / odbor:
Biology / Parasitology