Genetic diversity and demographic history of two endemic shrimp species

Divyadharshini Shanthakrishnan

Diplomová práce

Abstract:

Dugustella valentina and Palaemon zariquieyi are shrimp species endemic to the Valencia region, Spain. Using a mitochondrial Cytochrome c oxidase subunit-I (COI) marker sequenced from individuals of both species collected at multiple localities the demographic patterns of these species were evaluated by determining the nucleotide diversity, haplotype diversity, haplotype networking, and Bayesian Coalescence inference. Also, the connectivity between populations from different localities was assessed using landscape genetic tools and the level of genetic diversity was compared with the samples from previous years. The results suggest the level of genetic diversity is fairly high but appears to be reduced over years for D. valentina while P. zariquieyi has similar patterns with the reference populations. In both species, the gene flow and connectivity between localities is substantial, possibly mediated by irrigation channels or translocation by floods, birds, or humans. Additionally, the existence of a distant haplotype in D. valentina was subjected to phylogenetic analysis based on 16s rRNA and COI markers revealing the occurrence of two distinct lineages due to secondary contact or possibly by mitochondrial capture from unrecognized or extinct lineage. Such evaluations of these understudied species can help us understand the population structure and anthropogenic pressure on them to implement appropriate management plans for their conservation.

Keywords

Dugustella valentina, Palaemon zariquieyi, phylogeny, genetic diversity, 16s rRNA, landscape genetics, COI

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 22. 4. 2022

Obhajoba závěrečné práce

• Vedoucí: Mgr. Kristýna Eliášová

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Magisterský studijní program / specializace:
Wildlife and Livestock Production, Management and Conservation / Wildlife Management and Conservation