Effect of GYY4137 on tomcat epididymal spermatozoa stored at 5 °C during 7 days

Bc. Daniel Fuchs

Diplomová práce

Abstract:

Several recent studies investigated the usage of slow-releasing H2S donor GYY4137 as in vitro therapeutic agent against oxidative stress. Most of those studies observed antioxidant properties of this compound when administered on different types of cells and tissues submitted to oxidative stress. However, to date, the effect of this compound on tomcat spermatozoa were not investigated. Based on those observations, the hypothesis of this thesis was that GYY4137 may possess antioxidant properties and thus may protect tomcat spermatozoa against oxidative stress, which frequently arises during sperm storage. Therefore, the aim of this thesis was to test the effect of GYY4137 supplementation on the sperm parameters such as motility, plasma membrane integrity, acrosomal integrity and total antioxidant capacity. The semen of 7 tomcats was treated with and without two different concentrations of GYY4137 (0.5 mM; 1 mM) and evaluated during 7 days at 5°C. Consistent with previous studies, cool storage at 5 °C negatively affects tomcat sperm motility and acrosome integrity. Moreover, the results from this thesis provide evidence that GYY4137 showed antioxidant properties after 7 days of sperm storage. However, both at 0.5 and 1 mM, GYY4137 did show neither beneficial nor detrimental effects on cat epididymal sperm parameters. In conclusion, at the concentrations tested in this study, GYY4137 did not prove to be beneficial in enhancing the lifespan of tomcat epididymal spermatozoa stored at 5 °C for 7 days.

Keywords

oxidative stress, spermatozoa, tomcat, ROS, hydrogen sulphide, GYY4137

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 26. 4. 2021

Obhajoba závěrečné práce

• Vedoucí: Dott. Ric. Eliana Pintus
• Oponent: Adéla Grieblová, externi

Česká zemědělská univerzita v Praze

Fakulta agrobiologie, potravinových a přírodních zdrojů

Magisterský studijní program:
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