Preparation of antibodies to determine the association of mitoribosomal complexes with mitochondrial membrane

Laura ZELLNER

Bakalářská práce

Abstract:

The assembly pathways of mitoribosomes, descendants of bacterial ancestors producing proteins encoded by vestigial mitochondrial genomes, remains largely unknown. To shed light on structural features of a precursor of the highly divergent small mitoribosomal subunits (mtSSU) from Trypanosoma brucei, called mtSSU assemblosome, recently characterized by cryoEM, we raised antibodies against two assembly factors present in the complex and against two subunits of the mature mtSSU. We used the antibodies in pilot experiments to determine whether the assemblosome associates with the inner mitochondrial membrane.

Abstract:

The assembly pathways of mitoribosomes, descendants of bacterial ancestors producing proteins encoded by vestigial mitochondrial genomes, remains largely unknown. To shed light on structural features of a precursor of the highly divergent small mitoribosomal subunits (mtSSU) from Trypanosoma brucei, called mtSSU assemblosome, recently characterized by cryoEM, we raised antibodies against two assembly factors present in the complex and against two subunits of the mature mtSSU. We used the antibodies in pilot experiments to determine whether the assemblosome associates with the inner mitochondrial membrane.

Keywords

mitochondrial ribosome, mitoribosome, antibody production, trypanosoma brucei, recombinant protein, antigen, mitochondria, subcellular fractionation

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 3. 12. 2020

Obhajoba závěrečné práce

• Vedoucí: Mgr. Ondřej Gahura, Ph.D.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Bakalářský studijní program / obor:
Biochemistry / Biological Chemistry