Assessment of Genetic Diversity of Solanum sessiliflorum Dunal by application of ISSR markers

B.Sc. Sokhour May

Master's thesis

Abstract:

Solanum sessiliflorum Dunal is an Amazonian crop species and one of the valuable crops in Solanaceae family serving main roles in Latin American cuisine soft drink and mainly in ethnomedicine. This species contains variations in fruit shape and size; however the research on genetics is limited. Among the molecular markers Inter Simple Sequence Repeat (ISSR) provides simple technique produces more polymorphic loci and its cost is inexpensive. It has been used on many Solanum spp. such as S. tuborosum S. nigrum and Lycopersicon esculentum etc. A total number of 248 samples divided into 40 populations were collected from 3 regions in Peru. Based on fruit shape it was divided into five: trangular heart oblong flat and round. DNA isolation was done by CTAB method and six primers were selected from 30 primers after optimum annealing temperature of PCR. They provided several clearly reproducible bands. In total they generated 6143 bands with 133 amplified loci while 131 were polymorphic bands. Shannon Index within populations was 0.1398 and Nei genetic diversity was 0.0905 in average. Among populations Shannon Index showed higher value to 0.2783 accompanying with Nei Genetic distance which was 0.1672 especially gene flow acquired high number 0.5871 and GST got 0.7277. The analysis of molecular variance (AMOVA) illustrated 72% of genetic diversity within populations 21% among populations and only 7% among regions. The hierarchical clustering (UPGMA) of populations tend to distinguish LP11 LP12 LP13 and LP14 from the rest. Another dendrogram of all the samples differentiated neither according to the populations nor regions. The factorial analysis strongly supported this dendrogram except samples from LP1 and LP3 which were almost together. Even though the evaluation of phenotypes founded only on fruit shape ISSR helped to obviously investigate the genetic variation within this species. This present study will stand as the basic part for further research in genetics particularly in breeding program.

Keywords

Solanum sessiliflorum, ISSR, genetic diversity, fruit shape

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 18. 4. 2016

Obhajoba závěrečné práce

• Vedúci: Ing. Petra Hlásná Čepková, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Master programme / odbor:
Tropical Agriculture / Tropical Crop Management and Ecology