Optimization of Single-molecule FRET Experiments to observe the Conformational Dynamics in Sec Translocon During Protein Transport. – Lorin LEHNER
Lorin LEHNER
Bachelor's thesis
Optimization of Single-molecule FRET Experiments to observe the Conformational Dynamics in Sec Translocon During Protein Transport.
Mechanism of energy conversion in ATP driven molecular machines: single-molecule observation of conformational changes in sec translocon during protein transport
Abstract:
In this thesis, single molecule experiments were optimized for the detection of conformational changes of the lateral gate in translocon. Fluorescence Correlation Spectroscopy was performed to find the best suited acceptor dye in single molecule Förster Resonance Energy Transfer (smFRET). The smFRET data was then analyzed by the open-source python notebook FRETbursts and the different fluorescence …moreAbstract:
In this thesis, single molecule experiments were optimized for the detection of conformational changes of the lateral gate in translocon. Fluorescence Correlation Spectroscopy was performed to find the best suited acceptor dye in single molecule Förster Resonance Energy Transfer (smFRET). The smFRET data was then analyzed by the open-source python notebook FRETbursts and the different fluorescence …more
Language used: English
Date on which the thesis was submitted / produced: 9. 5. 2024
Thesis defence
- Supervisor: Mgr. Tomáš Fessl, Ph.D.
Citation record
ISO 690-compliant citation record:
LEHNER, Lorin. \textit{Optimization of Single-molecule FRET Experiments to observe the Conformational Dynamics in Sec Translocon During Protein Transport.}. Online. Bachelor's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2024. Available from: https://theses.cz/id/vb7lf9/.
The right form of listing the thesis as a source quoted
LEHNER, Lorin. Optimization of Single-molecule FRET Experiments to observe the Conformational Dynamics in Sec Translocon During Protein Transport.. České Budějovice, 2024. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceBachelor programme / field:
Biochemistry / Biological Chemistry
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Bulánová, L.
11/5/2024