Příprava rekombinantního proteinu kalcium-dependentní proteinkinázy 5 prvoka Babesia divergens

Zuzana CÍSAŘOVÁ

Bachelor's thesis

Abstract:

Babesia divergens, an Apicomplexan parasite, infects both bovines and humans, resulting in babesiosis. The life cycle of B. divergens is complex, with various developmental events coordinated by a calcium signaling pathways that are regulated by Ca2+-dependent protein kinases (CDPKs). While recent research has highlighted the crucial role of CDPKs in apicomplexan infectivity, including motility, host cell invasion, and transmission into the vector, Babesia CDPKs remain poorly characterized. This thesis focuses on BdCDPK5, a protein thus far uncharacterized with an unknown role in the Babesia life cycle. The recombinant BdCDPK5 protein was expressed and purified using the Escherichia coli expression system, representing a valuable tool for further studies such as biochemical characterization and 3D structural analysis. Additionally, polyclonal antibodies were generated and validated for their ability to recognize the BdCDPK5 molecule, enabling the detection of this enzyme using various immunomicroscopy applications.

Kľúčové slová

Babesia divergens, kalcium-dependentní proteinkinázy, CDPKs, BdCDPK5, purifikace, polyklonální protilátky

Jazyk práce: Czech

Datum vytvoření / odevzdání či podání práce: 12. 4. 2024

Obhajoba závěrečné práce

• Vedúci: RNDr. Marie Jalovecká, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Bachelor programme / odbor:
Biology / Biology