NMR studie retrovirové proteasy se zaměřením na vazbu jednovláknových nukleových kyselin k C-terminální doméně

Bc. Veronika Motáčková, Ph.D.

Master's thesis

NMR study of a retroviral protease focused on single-stranded nucleic acid binding to the C-terminal domain

Abstract:

Applications of nuclear magnetic resonance (NMR) are not limited to structure determination. Via NMR it is possible to study intermolecular interaction as well. This work presents an example of an NMR study of DNA-protein interaction, namely the binding of ss-DNA 20-mer to the C-terminal domain of the inMIA protease. The principle of the sequential assignment of residues to the protein backbone is described. The results are used to interpret titration experiments used for the searching for the individual residues mostly affected by the interaction. The binding model is proposed.

Keywords

inMIA14PR, CT3, ss-DNA 20-mer, sequential assignment, titration, binding model, simulation

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 16. 5. 2008

Obhajoba závěrečné práce

• Obhajoba proběhla 17. 6. 2008
• Vedúci: prof. Mgr. Lukáš Žídek, Ph.D.

Masaryk University

Faculty of Science

Master programme / odbor:
Biochemistry / Biomolecular Chemistry