Alkaloidy Narcissus pseudonarcissus cv Dutch Master (Amaryllidaceae) a jejich potenciální využití v terapii Alzheimerovy choroby

Bc. Jana Bartošková

Master's thesis

Alkaloids of Narcissus pseudonarcissus cv Dutch Master and their potential use in the therapy of Alzheimer's disease

Abstract:

Tato práce se zabývá výzkumem alkaloidů lykoreninu a odulinu z čeledi Amaryllidaceae, druhu Narcissus pseudonarcissus, jejich izolací, identifikací a biologickou aktivitou. Zmíněné alkaloidy byly testovány s cílem nalezení nových účinnějších inhibitorů cholinesteráz pro léčbu Alzheimerovy choroby. Inhibitory cholinesteráz jsou aktuálně jedinými léky používanými na zpomalení tohoto degenerativního onemocnění. Alkaloidy lykorenin a odulin byly podrobeny strukturní analýze prostřednictvím GC/MS a NMR, následně inhibičnímu testu vůči acetylcholinesteráze a butyrylcholinesteráze charakterizovaným hodnotou IC50, byl určen typ inhibice a nakonec rozdělovacíkoeficient látek v systému 1-oktanol:voda.

Abstract:

This thesis deals with research of alkaloids lycorenine and oduline from family Amaryllidaceae, species of Narcissus pseudonarcissus, their isolation, identification and biological activity. These alkaloids were tested in order to find the most effective inhibitors of cholinesterases for treating Alzheimer's disease. The inhibitors of cholinesterases represent the only drugs that are currently used for deceleration of the disease. In the first stage of the process described, the structures of alkaloids, lycorenine and oduline were analysed using GC/MS and NMR. The analysis was then followed by testing of the inhibitory activity against acetylcholinesterase and butyrilchulinesterase characterized by IC50. In the upshot, the inhibition type and the partition coefficient of substances in the system of 1-octanol:water were determined.

Keywords

AD, Amaryllidaceae, cholinesterázy, inhibitory cholinesteráz

Language used: Czech

Date on which the thesis was submitted / produced: 13. 5. 2016

Thesis defence

• Date of defence: 7. 6. 2016
• Supervisor: Mgr. Šárka Štěpánková, Ph.D.

University of Pardubice

Faculty of Chemical Technology

Master programme / field:
Special Chemical and Biological Programmes / Analysis of Biological Materials