Chemical analysis of garlic essential oils (Allium sativum L.) from different countries of origin

B.Sc. PRINCE OWUSU KODOM, BSc

Diplomová práce

Abstract:

Allium sativum, likewise called garlic, is used widely around the world in both cooking and medicine. The chemical makeup of the essential oils from Allium Sativum was examined in the current study. The aromatic compounds extracted from five countries of origin-grown Allium sativum bulbs, namely; the Czech Republic, China, Spain, Slovakia and Italy, were obtained by laboratory hydro-distillation method using a Clevenger apparatus. The resulting essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). Crushing-raw fresh garlic clove were used to analyse garlic’s volatiles profiles. Volatiles of headspace in the five different samples as mentioned above were extracted by a Solid-Phase Microextraction (SPME) and analyzed with GC-MS. Many volatile compounds were acknowledged in the fresh garlic using the SPME-GC-MS method. Volatile compounds found included Diallyl disulfide, Diallyl sulfide, Di-2-propenyl trisulfides, 2-Propenylmethyltrisulfide, Allyl (E)-1-propenyldisulfide, 3-Vinyl-1,2-dithiacyclohex-4-ene, 2-Vinyl-4H-1,3-dithiine, 4H-1,2,3-Trithiine, Methyl (E)-1-propenyl disulfane, and 2-Propenylmethyldisulfide. Antibacterial activity of Allium savitum essential oils was determined against Staphylococcus aureus by oxacillin using the method of new broth microdilution volatilization. The present work’s objective was to measure the variation in colour change and pH of fresh garlic. The results showed that the Hue (colour of garlic determined by the amount of carotenoids present in the garlic) was highest in the China garlic sample, followed by Slovakia, Czech Republic, Italy and Spain accordingly. Finally, in terms of total colour change, China was the best, followed by Italy, the Czech Republic, Slovakia, and Spain.

Keywords

Garlic (Allium sativum), Volatile substance, Antibacterial activity, Colour, pH

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 25. 4. 2023

Obhajoba závěrečné práce

• Vedoucí: doc. Klára Urbanová, Ph.D.
• Oponent: Pavel Nový, Ph.D.

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Magisterský studijní program:
Agri-food Systems and Rural Development