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Bc. Petra Paclíková

Master's thesis

Funkční analýza buněk bez proteinu Dishevelled

Functional analysis of cells lacking Dishevelled
Abstract:
Tato diplomová práce ve své teoretické části shrnuje poznatky o Wnt signalizaci a proteinu Dishevelled, který je klíčovou komponentou této signalizace. V praktické části je využit buněčný model odvozený s buněčné linie HEK293, který byl vytvořen metodou CRISPR/Cas9 a chybí mu všechny isoformy proteinu DVL. Tento model by měl pomoci při studiu vlivu absence proteinu Dishvelled na Wnt signalizaci.
Abstract:
This thesis summarizes existing findings about Wnt signalling and protein Dishevelled that is a key component of these signalling pathways. In practical part, cell line model derived from HEK293 cells that was created by CRISPR/Cas9 and lacks all isoforms of DVL was used. This cell line should help to study influence of Dishevelled absence on Wnt signalling.
 

Keywords

Dishevelled Wnt/β-kateninová signaliazce Wnt/β-catenin signalling Wnt/PCP LRP5/6 ROR CRISPR/Cas9
 
Language used: English
Date on which the thesis was submitted / produced: 10. 5. 2016
Identifier: https://is.muni.cz/th/glm0o/

Thesis defence

  • Date of defence: 6. 6. 2016
  • Supervisor: Mgr. Ondřej Bernatík, Ph.D.

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Masaryk University

Faculty of Science

Master programme / field:
Experimental Biology / Molecular Biology and Genetics

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