In vitro production of secondary metabolites from Pseuderanthemum palatiferum

Kryštof Knejp

Bachelor's thesis

Abstract:

Pseuderanthemum palatiferum is an Acanthaceae plant used in Vietnamese and Thai traditional medicine and has been proven to have (at least some) anti-bacterial, anti-fungal, anti-inflammatory, anti-oxidative, anti-diabetic, anti-thrombotic, analgesic, bradicardic, hypotensive, cancer-inhibiting, gastric ulcer preventing and acetylcholinesterase activity inhibiting physiological effects. Which secondary metabolites are responsible for the mentioned physiological capabilities has not yet been revealed. In vitro cultivation of Pseuderanthemum palatiferum was optimalized and plantlets and callus were successfully micropropagated. The plantlets were then oven-dried and subjected to GC-MS analysis, based on which 25 unique chemical compounds were detected and identified. Dopamine and noradrenaline, which were among the identified compounds, were not previously reported to be present in Pseuderanthemum palatiferum. ‘Precursor feeding’ is the practice of supplementing culture medium with a precursor of a specific compound or group of compounds in order to increase their production in the plant tissues. Precursor feeding of Pseuderanthemum palatiferum plantlets and callus cultures was conducted using different concentrations of L-tyrosine in culture media – 25, 50, 100 and 200 mg.l-1. After 6 weeks of cultivation on medium supplemented with tyrosine, the plantlets and calli were oven-dried and subjected to a GC-MS analysis.

Keywords

Pseuderanthemum latifolium, catecholamines, medicinal plant, plant tissue culture, SouthEast Asia

Language used: English

Date on which the thesis was submitted / produced: 15. 5. 2020

Thesis defence

• Supervisor: Ing. Olga Leuner, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Bachelor programme / field:
Agricultural Specialization / Agriculture in Tropics and Subtropics