Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation – Johannes GRAHAMMER
Johannes GRAHAMMER
Bachelor's thesis
Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation
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Novel agitation module for freeze substitution
Abstract:
The quality of biological samples for Transmission Electron Microscopy prepared using High Pressure Freezing and Freeze Substitution with a novel agitation module invented by Helmuth Goldhammer and Siegfried Reipert was compared to Cells prepared without agitation and the Freeze Substitution protocol by Obornik et al. The amount of extraction was quantified via immunolabelling of RuBisCO inside the …moreAbstract:
The quality of biological samples for Transmission Electron Microscopy prepared using High Pressure Freezing and Freeze Substitution with a novel agitation module invented by Helmuth Goldhammer and Siegfried Reipert was compared to Cells prepared without agitation and the Freeze Substitution protocol by Obornik et al. The amount of extraction was quantified via immunolabelling of RuBisCO inside the …more
Language used: English
Date on which the thesis was submitted / produced: 14. 5. 2018
Thesis defence
- Date of defence: 18. 6. 2018
- Supervisor: RNDr. Marie Vancová, Ph.D.
Citation record
ISO 690-compliant citation record:
GRAHAMMER, Johannes. \textit{Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation}. Online. Bachelor's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2018. Available from: https://theses.cz/id/zv9e23/.
The right form of listing the thesis as a source quoted
GRAHAMMER, Johannes. Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation. Č. Budějovice, 2018. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceBachelor programme / field:
Biochemistry / Biological Chemistry
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