Assessment of the validity of the luminescence toxicity testing

Maria Dolgaya

Bakalářská práce

Abstract:

This bachelor thesis is focusing on the evaluation of the source of Aliivibrio fischeri (formerly Vibrio fischeri) on the validity of luminescent bacteria testing. The theoretical part of the work deals with the current state in the field of acute ecotoxicity measurements in the aquatic environments, relative released legislations and available standardized test protocols. The practical part is performed on the selected freshwater pollutants using the standardized marine luminescent bacteria A. fischeri. According to the results obtained from the experimental part performance carried out in laboratory conditions in compliance with the relevant ISO 11348-3:2007 standard, the most stable, precise, and cost-effective testing bacteria sources are LUMIStox© (Germany) and Biolight (Belgium). The difference between the results obtained from testing on different luminescent bacteria suppliers shows that the source of bacteria has a slight effect on the final results depending on the required conditions and the procedure required by the suppliers. This fact should be taken into consideration during test performance.

Keywords

Luminescence, Light emission, Aliivibrio fischeri, Acute toxicity, Water, Pollutant, Validity

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 2. 4. 2023

Obhajoba závěrečné práce

• Obhajoba proběhla 23. 5. 2023
• Vedoucí: Ing. Lenka Wimmerová, MSc Ph.D.
• Oponent: Ljuba Zídková, Ing., externi

Česká zemědělská univerzita v Praze

Fakulta životního prostředí

Bakalářský studijní program:
Environmental Engineering