Cytochemical methods extending sperm diagnosis

Mgr. Toghrul Naghiyev, B.Sc.

Master's thesis

Abstract:

In this study, we propose the utilization of silver nitrate staining to enhance the analysis of the morphological structures of spermatozoa. By incorporating this technique, we anticipated obtaining valuable insights into previously unexplored morphological aspects. The staining process has the potential to reveal intricate details and structures of sperm, which are indicative of sperm function and fertilization capacity. Our method is based on reaction of argyrophilic proteins with silver nitrate combined with enhancing effect of sodium thiosulphate and additional reduction by combination of ferric chloride, oxalic acid and hexacyanoferrate In comparison to other recently published papers and widely used methods our approach looks promising when it comes to demonstration of morphological structures within spermatozoa. Our research seeks to establish methods enabling in next research a correlation between the observed morphological variations and the potential for successful fertilization. These findings could contribute to the development of enhanced diagnostic tools for assessing male fertility and assist in personalized reproductive interventions. Ultimately, this research may have far-reaching implications for improving the diagnosis and treatment of male infertility, as well as guiding assisted reproductive technologies towards higher success rates.

Keywords

morfologie lidských spermií, techniky asistované reprodukce, fertilizační potenciál, mužská neplodnost

Language used: English

Date on which the thesis was submitted / produced: 5. 1. 2024

Thesis defence

• Date of defence: 9. 2. 2024
• Supervisor: prof. MUDr. Pavel Trávník, DrSc.
• Reader: doc. Ing. Michal Ješeta, Ph.D.

Masaryk University

Faculty of Science

Master programme / field:
Molecular and Cell Biology / Molecular and Cell Biology