In vitro Induced Mitotic Polyploidy in Anemone sylvestris L.

Bc. Andrea Dvořáková

Diplomová práce

Abstract:

Snowdrop anemone (Anemone sylvestris L.) is ornamental plant from the family Ranunculaceae that can be used as outdoor and indoor plant. It is native to Eurasia and is among the spring-blooming anemone.The main objective of this thesis was to obtain octoploid (2n=64) plants of snowdrop anemone from tetraploid (2n=32) plants by using induced mitotic polyploidization in vitro.For polyploidization shoot clumps were used, that were exposed to the effect of oryzalin as antimitotic agent. The plants were cultivated on MS medium free of plant growth regulators (PGRs) with the addition of oryzalin in concentration 1, 5 and 10 micromoles with an exposure period 6 and 10 weeks. After the shoot clumps were cultivated on MS medium without PGRs and they were multiplied several times during 2 months. The DNA-ploidy levels were measured using flow cytometry. The polyploidization efficiency was 1.67 % and 3 octoploid plants were obtained. The successful concentration of oryzalin was 1 micromole and exposure period 6 and 10 weeks.These obtained octoploid plants (2n=64) were qualitatively (colour of leaves, shape of leaves), quantitatively (dry matter, leaf area), morphologically (number of leaves, length of petioles, length of leaves, height of plant) and molecularly (number of amplified loci, length of amplicons, number of unique fragments and length of their amplicons) different to tetraploid and diploid plants. Induced mitotic polyploidization in vitro can be successfully used in breeding programs of ornamental plants, including Anemone sylvestris.

Keywords

Anemone sylvestris, flow cytometry, in vitro, octoploid, oryzalin, Ranunculaceae, tetraploid

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 26. 4. 2017

Obhajoba závěrečné práce

• Vedoucí: doc. Dr. Ing. Eloy Fernández Cusimamani

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Magisterský studijní program / obor:
Tropické zemědělství / Tropical Crop Management and Ecology