Izolace a charakterizace kmenů z nové skupiny acidofilních aktinobakterií.

Jan Kotyk

Bachelor's thesis

Abstract:

Previously done research on acidophilic bacteria from waterlogged acidic forest soils in Třeboň region found a new deeply branched cluster of Actinobacteria. The Trebon Clade has had one species described yet, Trebonia kvetii, within a family Treboniaceae. Due to this discovery, another research needed was to support this observation by isolation of relatives of this new family. Actinobacteria are an interesting group of bacteria for their importance in the cycles of elements, decomposition of organic matter and xenobiotics, and the ability to create secondary metabolites. They are so far the main source of currently used antibiotics. They can live under extreme conditions, which may be limiting for other organisms. Two sampling points were chosen for isolation of noval strains from soil. Locations near the towns of Zakopaná and Třeboň had similar soil properties, thus the samples of soil for isolation were collected to obtain strains with wider adaptations and physiology. Locations are characteristic mostly by their low soil pH. At both sites, the presence of Treboniaceae family was proved by 16S rRNA gene sequencing of the whole soil community. In this study, after plating, transferring, DNA extraction, and PCR preparation, 17 strains of Actinobacteria belonging to genera Actinospica, Nocardia....were found to be good enough for the sequencing and further creation of phylogenetic trees to get an idea of their genetic origin.

Keywords

Actinobacteria, acidic soil, isolation of bacteria

Language used: English

Date on which the thesis was submitted / produced: 22. 4. 2022

Thesis defence

• Supervisor: doc. RNDr. Markéta Marečková, Ph.D.

Czech University of Life Sciences Prague

Faculty of Agrobiology, Food and Natural Resources

Bachelor programme / field:
Sustainable Use of Natural Resources / Sustainable Use of Natural Resources