Faktory ovlivňující regulaci expresi genu ferA bakterie Paracoccus denitrificans – Bc. Barbora Šatná
Bc. Barbora Šatná
Bachelor's thesis
Faktory ovlivňující regulaci expresi genu ferA bakterie Paracoccus denitrificans
Factors affecting the regulation of ferA gene expression in the bacterium Paracoccus denitrificans
Abstract:
K detekci genové exprese ferA v bakterii Paracoccus denitrificans byl využit reportérový enzym β-galaktosidasa. Experimenty byly prováděny v aerobním médiu v prostředí s nízkou koncentrací železa a v přítomnosti Fe(III)-(DHB)3. Postupná limitace železa pro bakterii znamenala zpomalení jejího růstu. Obdobné vlastnosti vykazoval kmen s přerušeným ferA. Vypočtené aktivity β-galaktosidasy v závislosti …moreAbstract:
For the detection of gene expression ferA in bacteria Paracoccus denitrificans reporter enzyme β-galactosidase was used. Experiments were performed in an aerobic medium in an environment with low concentration of iron and in the presence of Fe(III)-(DHB)3. Gradual limitation of iron caused reduction of the growth of bacteria. Similar behaviour was observed at strain with interrupted ferA. Depending …more
Language used: Czech
Date on which the thesis was submitted / produced: 9. 2. 2012
Identifier:
https://is.muni.cz/th/mdjqs/
Thesis defence
- Date of defence: 12. 6. 2012
- Supervisor: prof. RNDr. Igor Kučera, DrSc.
Citation record
Full text of thesis
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Institution archiving the thesis and making it accessible: Masarykova univerzita, Přírodovědecká fakultaMasaryk University
Faculty of ScienceBachelor programme / field:
Applied Biochemistry / Applied Biochemistry
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