In vitro growth-inhibitory effect of Philippine medicinal plants traditionally used to cure diarrhoea against intestinal bacterial pathogens

Bc. Barbora Fišerová

Diplomová práce

Abstract:

Infectious diarrhoea is serious public health problem worldwide, mainly in developing countries, including Philippines. Diarrhoeal disease is the third leading cause of death worldwide with 2.2 million deaths. Plants used in traditional medicine for treatment of diarrhoea are tested for antimicrobial activity, because of increasing resistance of bacteria to antibiotics, and in countries such as Philippines antibiotics can be hard to reach or expensive for people from rural areas, where 80 % of them is still dependent on indigenous medicine.In this study, 19 species were collected, air-dried and tested for their antimicrobial effects by broth microdilution method. Six of them showed positive results against 5 out of 8 tested representatives of pathogenic bacteria. Artocarpus camansi species showed the highest antibacterial effect against Clostridium perfringens (MIC = 128 μg/mL) and Clostridium difficile (MIC = 128 μg/mL). Significant anti-bacterial effects exhibited also Artocarpus blancoi against Enterococcus faecalis (MIC = 128 μg/mL), Acalypha grandis against Vibrio parahaemolyticus (MIC = 256 μg/mL), and Diplazium esculentum and Picrasma javanica produced growth-inhibitory action against Escherichia coli with MICs 256 and 512 μg/mL, respectively. Carmona retusa possessed moderate effect against Vibrio parahaemolyticus (512 μg/mL).These results suggest above-mentioned species as perspective plant materials for development of pharmaceutical and food applications effective for treatment of diarrhoea. However, further research focused on identification of their antimicrobial principles and evaluation of their safety will be necessary.

Keywords

diarrhoea, Philippines, medicinal plants, antibacterial extracts

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 26. 4. 2019

Obhajoba závěrečné práce

• Vedoucí: prof. Ing. Ladislav Kokoška, Ph.D.

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Magisterský studijní program / obor:
Tropické zemědělství / Tropical Crop Management and Ecology