Development of an efficient slow-growth method for the In vitro storage of garlic (Allium sativum)

Yehor Nykyforov

Diplomová práce

Abstract:

The present study aimed at developing an efficient slow-growth protocol for the medium-term storage of garlic (Allium sativum) by testing the effects of various osmotic agents at various concentrations (sorbitol 20-60 g/l, mannitol 20-60 g/l, and sucrose 30-150 g/l) and plant growth regulators (chlormequat chloride (CCC) at 200-600 mg/l and abscisic acid (ABA) at 1-5 mg/l). Two cultivation temperatures, 5±1°C, and 18±1°C were also tested to observe their influence on the growth of the plants. Full-strength and 1/2 concentrated MS (Murashige and Skoog (1962) were used as basal culture media. Growth and development characteristics after 5-month slow-growth storage showed that garlic plantlets stored either on a concentration of 5 mg/l ABA in combination with full-strength MS medium, sucrose at 10% in half MS medium, or sorbitol at 4% in full MS, all at 5°C±1°C cultivation temperature prove to be most effective in reducing the growth of in-vitro plantlets. After the storage period, plantlets from these treatments were transferred to recovery media. Results showed that garlic plantlets stored on 1/2 MS medium supplemented by 10% sucrose had the strongest growth capacity. Relatively high regenerative abilities were also shown by plants stored in MS nutrient medium containing 4% sorbitol. As for the effect of ABA 5 mg/l in combination with full-strength MS medium on the regenerative capacity of garlic, results showed that the growth was weak, suggesting a need for long-term rehabilitation of garlic plants after the influence of this growth-regulating substance.

Keywords

Slow-growth storage, Alium sativum, Medium-term conservation, Osmotic agents, Plant growth regulators, Mannitol, Sorbitol, Sucrose, Chlormequat chloride (CCC), Abscisic acid (ABA)

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 21. 4. 2022

Obhajoba závěrečné práce

• Vedoucí: doc. Ing. Hynek Roubík, Ph.D.

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Magisterský studijní program:
Agri-food Systems and Rural Development