Determination of in vitro antistaphylococcal effect of Cambodian essential oil-bearing plants using broth microdilution volatilization method

Ingrid Faltová

Diplomová práce

Abstract:

Staphylococcus aureus is pathogenic bacteria, which can cause life-threatening infec-tious diseases. Since the resistance to antibiotics still increases, there is a need to dis-cover new anti-infective agents. Plants provide rich source of secondary metabolites including essential oils possessing antimicrobial activity.Within this thesis in vitro growth inhibitory effect of Cambodian essential oil-bearing plants from Lauraceae and Zingiberaceae families were tested against 5 representatives of Staphylococcus aureus. Firstly, essential oils were distilled, subsequently, their an-tistaphylococcal activity was assayed using novel broth microdilution volatilisation method and the minimum inhibitory concentrations were determined. It was found, that three essential oils possessed certain degree of antibacterial activity in liquid or vapour phase. The most effective were Amomum pierreanum, which inhibited growth of 4 strains of S. aureus in broth medium with MIC 1024 μg/mL and Cinnamomum dimor-phandrum active against two strains in liquid phase and another two strains in vapour phase, both at MIC 1024 μg/mL. According to our results this method confirmed to be suitable for rapid simultaneous determination of antibacterial potential of EOs in the liquid and the vapour phase at different concentrations.A. pierreanum and C. dimorphandrum are suggested as prospective plant materials for further study regarding the isolation and identification of active compounds which are responsible for antistaphyloccocal activity. These findings indicate that further studies on chemical and biological properties of their active components should be performed.

Keywords

Antimicrobial activity, broth microdilution volatilization method, essential oils, Cambodian aromatic plants

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 27. 4. 2018

Obhajoba závěrečné práce

• Vedoucí: prof. Ing. Ladislav Kokoška, Ph.D.
• Oponent: Jan Tauchen, Ing.

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Magisterský studijní program / obor:
Tropické zemědělství / Tropical Crop Management and Ecology