Thin Nanostructured Film with Bioapplication Potential

Sanjay KUMAR

Disertační práce

Abstract:

The Ph.D. thesis aims to investigate, develop, and fabricate advanced nanostructured surfaces for (bio)sensors and detection applications. The primary outcome of the research is the development of a functional sensor transducer for the detection of specific anti-DbpA antibodies, typically produced by a living organism in response to Lyme disease infection, as well as for detecting live Borreliosis pathogens. The developed sensor is based on AgNPs/C:H:N:O plasma polymer nanocomposites. Silver (Ag) nanoparticles, generated by a gas aggregation source, are incorporated into a C:H:N:O plasma polymer matrix, which is deposited by magnetron sputtering of nylon 6.6. The surface of the AgNPs/C:H:N:O nanocomposite is functionalized with amino-rich -NH2 grafts and specific antibodies to achieve high specificity in detection. Anchored Ag nanoparticles propagate localized surface plasmon resonance (LSPR), optically responding to changes caused by immobilized targets near the nanoparticles. The sensitivity of the sensor transducer is enhanced by incorporating Cu nanoparticles, which contribute to Cu-Ag coupling modes responsible for the modulation of the LSPR bands. The achieved results pave the way for rapid, low-cost, sensitive platforms for detecting not only the antibodies/pathogens studied here but also potentially other targets.

Abstract:

The Ph.D. thesis aims to investigate, develop, and fabricate advanced nanostructured surfaces for (bio)sensors and detection applications. The primary outcome of the research is the development of a functional sensor transducer for the detection of specific anti-DbpA antibodies, typically produced by a living organism in response to Lyme disease infection, as well as for detecting live Borreliosis pathogens. The developed sensor is based on AgNPs/C:H:N:O plasma polymer nanocomposites. Silver (Ag) nanoparticles, generated by a gas aggregation source, are incorporated into a C:H:N:O plasma polymer matrix, which is deposited by magnetron sputtering of nylon 6.6. The surface of the AgNPs/C:H:N:O nanocomposite is functionalized with amino-rich -NH2 grafts and specific antibodies to achieve high specificity in detection. Anchored Ag nanoparticles propagate localized surface plasmon resonance (LSPR), optically responding to changes caused by immobilized targets near the nanoparticles. The sensitivity of the sensor transducer is enhanced by incorporating Cu nanoparticles, which contribute to Cu-Ag coupling modes responsible for the modulation of the LSPR bands. The achieved results pave the way for rapid, low-cost, sensitive platforms for detecting not only the antibodies/pathogens studied here but also potentially other targets.

Keywords

plasma polymer, plasma polymerization, C, H, N, O thin film, nylon, nanoparticles (Ag, Cu), nanostructures, nanocomposite, surface functionalization, magnetron sputtering, annealing, adhesion, immobilization, sensors, antibacterial, Lyme disease, Borrelia, plasmonic absorption, localized surface plasmon resonance (LSPR), plasma-assisted deposition, enhanced detection

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 23. 7. 2024

Obhajoba závěrečné práce

• Vedoucí: prof. RNDr. Vítězslav Straňák, Ph.D.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Doktorský studijní program / obor:
Biophysics / Biophysics