Cloning and Expression of two selected Antigenic Proteins and their characterization as diagnostic markers against Relapsing Fever Disease

Esma ÖZCAN

Bakalářská práce

Cloning and Expression of selected Antigenic Proteins and their characterization as diagnostic markers against Relapsing Fever Disease

Abstract:

This bachelor thesis focuses on the cloning, expression, and characterization of two selected antigenic proteins, Lactate Dehydrogenase (LD) and Human Complement Plasminogen A (hcpA), to evaluate their potential as diagnostic markers against Relapsing Fever Disease caused by Borrelia duttonii. Relapsing Fever is a neglected tropical disease with significant public health implications, yet it remains under-researched, leading to challenges in effective diagnosis and treatment. This study addresses the urgent need for new diagnostic tools by investigating the molecular properties of LD and hcpA, assessing their suitability as reliable markers for detecting B. duttonii infections. The successful amplification and cloning of these genes pave the way for further research, although significant challenges were encountered in the expression phase, particularly concerning protein stability and solubility. This research contributes to the growing body of knowledge on diagnostic markers for infectious diseases and highlights the potential of these proteins to improve diagnostic accuracy for Relapsing Fever.

Abstract:

This bachelor thesis focuses on the cloning, expression, and characterization of two selected antigenic proteins, Lactate Dehydrogenase (LD) and Human Complement Plasminogen A (hcpA), to evaluate their potential as diagnostic markers against Relapsing Fever Disease caused by Borrelia duttonii. Relapsing Fever is a neglected tropical disease with significant public health implications, yet it remains under-researched, leading to challenges in effective diagnosis and treatment. This study addresses the urgent need for new diagnostic tools by investigating the molecular properties of LD and hcpA, assessing their suitability as reliable markers for detecting B. duttonii infections. The successful amplification and cloning of these genes pave the way for further research, although significant challenges were encountered in the expression phase, particularly concerning protein stability and solubility. This research contributes to the growing body of knowledge on diagnostic markers for infectious diseases and highlights the potential of these proteins to improve diagnostic accuracy for Relapsing Fever.

Keywords

Borrelia duttonii, relapsing fever, soft ticks, Ornithodoros moubata, hcpA, LD, diagnostic marker

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 19. 8. 2024

Obhajoba závěrečné práce

• Vedoucí: Ryan Oliver Marino Rego, Ph.D.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Bakalářský studijní program / obor:
Biological Chemistry / Biological Chemistry