Studium dynamiky nukleových kyselin pomocí NMR spektroskopie jader C-13

Bc. Pavel Kadeřávek, Ph.D.

Master's thesis

Aspects of 13-C Relaxation Analysis and Application to Study Fast Motions in UUCG Hairpin Loop

Abstract:

The diploma thesis deals with the methods for studying fast internal dynamics of nucleic acids by carbon relaxation in NMR spectroscopy. The established procedures are based on some assumptions, namely that spin systems are isolated and the chemical shielding tensors are axially symmetric, that are not met for carbons in fully labelled nucleic acids. The thesis extends the method of Model-Free analysis to include the effects of the neighbouring nuclei and of the asymmetric chemical shielding tensor. Both the standard and the extended analyses were applied to fully C-13, N-15 labelled RNA hairpin that includes a UUCG loop. The comparison of the results of the two approaches revealed that the mentioned effects are not negligible and the analysis should consider them.

Keywords

NMR, C-13 relaxation, RNA dynamics

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 15. 5. 2008

Obhajoba závěrečné práce

• Obhajoba proběhla 17. 6. 2008
• Vedúci: doc. RNDr. Radovan Fiala, CSc.

Masaryk University

Faculty of Science

Master programme / odbor:
Biochemistry / Biomolecular Chemistry