The development and application of an analytical protocol for the isolation and quantification of indole-3-acetic acid and its derivates in plant material – Aleš Pěnčík
Aleš Pěnčík
Doctoral thesis
The development and application of an analytical protocol for the isolation and quantification of indole-3-acetic acid and its derivates in plant material
The development and application of an analytical protocol for the isolation and quantification of indole-3-acetic acid and its derivates in plant material
Abstract:
The aim of this thesis was to develop an analytical protocol suitable for the isolation and quantification of indole-3-acetic acid (IAA) and its derivatives. To achieve this, a specific immunoaffinity extraction procedure was developed and implemented into the protocol. The developed protocol was used for analysis of IAA conjugates in seeds of Christmas rose (Helleborus niger L.) Besides successful …moreAbstract:
Cílem práce bylo vyvinout analytický protokol pro izolaci a kvantifikaci konjugátů kyseliny 3-indolyloctové (IAA) v rostlinném materiálu, využívající immunoafinitní purifikaci a vysokoúčinnou kapalinovou chromatografii s tandemovou hmotnostní detekcí. Vyvinutý protokol byl využit pro stanovení IAA a sedmi jejích konjugátů s aminokyselinami v semenech vánoční růže (Helleborus niger L.). Tři z těchto …more
Language used: English
Date on which the thesis was submitted / produced: 24. 6. 2010
Thesis defence
- Date of defence: 24. 6. 2010
- Reader: Martin Fellner, Els Prinsen, Petre Dobrev
Citation record
ISO 690-compliant citation record:
PĚNČÍK, Aleš. \textit{The development and application of an analytical protocol for the isolation and quantification of indole-3-acetic acid and its derivates in plant material}. Online. Doctoral theses, Dissertations. Olomouc: Palacký University Olomouc, Přírodovědecká fakulta. 2010. Available from: https://theses.cz/id/kd7yvj/.
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Published in Theses:- Soubory jsou od 24. 6. 2010 dostupné: světu
Univerzita Palackého
Přírodovědecká fakultaDoctoral programme / field:
Biologie / Botanika
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