Role of queuosine tRNA modification in the parasitic protist \kur{Trypanosoma brucei}

Sneha Sunil KULKARNI, MSc.

Doctoral thesis

Abstract:

This study analyzes the queuosine (Q) tRNA modification and the enzyme responsible for Q formation, the tRNA guanine transglycosylase (TGT), in the parasitic protist \kur{Trypanosoma brucei}. We describe the interdependence of Q modification and tRNA subcellular trafficking, and how these two factors regulate codon biased translation. The effect of Q on translation has serious implications in the virulence of the parasite inside the mammalian host.

Abstract:

This study analyzes the queuosine (Q) tRNA modification and the enzyme responsible for Q formation, the tRNA guanine transglycosylase (TGT), in the parasitic protist \kur{Trypanosoma brucei}. We describe the interdependence of Q modification and tRNA subcellular trafficking, and how these two factors regulate codon biased translation. The effect of Q on translation has serious implications in the virulence of the parasite inside the mammalian host.

Keywords

Queuosine, tRNA guanine transglycosylase, tRNA modifications, tRNA traficking, mitochondria, synonymous codon usage

Language used: English

Date on which the thesis was submitted / produced: 1. 9. 2021

Thesis defence

• Supervisor: RNDr. Zdeněk Paris, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Doctoral programme / field:
Molecular and Cell Biology / Molecular and Cell Biology and Genetics