1H NMR profiling of extracts from common vegetables

Giorgi Goderdzishvili, BSc

Master's thesis

Abstract:

The objective of this study was to use nuclear magnetic resonance (NMR) spectroscopy to investigate the some of the nutrients found in fruits and vegetables, such as carbohydrates, and organic acids. 1H NMR spectroscopy is an effective technique for determining structure that requires small sample volumes and a short processing time. The findings of this study reveal specific information about carbohydrates in study materials, such as sucrose, fructose, glucose, and mono-disaccharides, as well as organic acids, malate, and citric acids. were measured in 35 vegetable and fruit samples, each sample revealing its own chemical characteristic, citrus fruits reveal citric acids in large amounts, which are basic components for their identification and defining their quality, lime, lemon reveals citric acids concentration (135,665 g/L and 148,635 g/L). The citric acid concentration was also found in strawberry, mango, tomato, grapefruit, pomegranate, and oroblanco. Garlic, grapes, and mango all have a high carbohydrate content, according to research. Data were entered into the statistical program Metaboanalist to obtain statistical analysis (Wishart research group at the University of Alberta, Canada, www.metaboanalyst.ca). This study goes over all of the chemicals mentioned above and their specific roles in food adulteration, authentication, and determining quality. The same rapid mechanism can be used in the aforementioned circumstances of the food industry; there are clear parallels between this research and another identification process revealed by 1H NMR spectroscopy for the identification of food chemicals. 1H NMR enables classification and differentiation not only between species but also within them and reveals limitless scientific capabilities.

Keywords

NMR, vegetables, chemical analysis, nutrients

Language used: English

Date on which the thesis was submitted / produced: 22. 4. 2022

Thesis defence

• Supervisor: doc. Ing. Jaroslav Havlík, Ph.D.

Czech University of Life Sciences Prague

Faculty of Agrobiology, Food and Natural Resources

Master programme:
Sustainable Agriculture and Food Security