Effect of pH and detergents on the activity of enzymes immobilized by the layer-by-layer approach – Julia WOLFMAYR
Julia WOLFMAYR
Bachelor's thesis
Effect of pH and detergents on the activity of enzymes immobilized by the layer-by-layer approach
Effect of pH and detergents on the activity of enzymes immobilized by the layer-by-layer approach
Abstract:
In this thesis, the immobilization of Horseradish peroxidase in polyelectrolyte multilayers prepared by the layer-by-layer approach is studied. The layers have been prepared using polystyrene sulfonate (PSS) and polyallylamine hydrochloride (PAH) dissolved in Tris-buffered saline at different pH levels and in the presence of sodium dodecyl sulfate (SDS). The activity of the immobilized enzyme was analyzed …moreAbstract:
In this thesis, the immobilization of Horseradish peroxidase in polyelectrolyte multilayers prepared by the layer-by-layer approach is studied. The layers have been prepared using polystyrene sulfonate (PSS) and polyallylamine hydrochloride (PAH) dissolved in Tris-buffered saline at different pH levels and in the presence of sodium dodecyl sulfate (SDS). The activity of the immobilized enzyme was analyzed …more
Language used: Czech
Date on which the thesis was submitted / produced: 14. 12. 2023
Thesis defence
- Supervisor: RNDr. Ján Štěrba, Ph.D.
Citation record
ISO 690-compliant citation record:
WOLFMAYR, Julia. \textit{Effect of pH and detergents on the activity of enzymes immobilized by the layer-by-layer approach}. Online. Bachelor's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2023. Available from: https://theses.cz/id/ngcrvg/.
The right form of listing the thesis as a source quoted
WOLFMAYR, Julia. Effect of pH and detergents on the activity of enzymes immobilized by the layer-by-layer approach. České Budějovice, 2023. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceBachelor programme / field:
Biochemistry / Biological Chemistry
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Bulánová, L.
15/12/2023