Předpověď elučních dat v gradientech mobilní fáze na kolonách s povrchově porézními částicemi

Bc. Marie Růžičková

Master's thesis

Prediction of gradient elution data on columns with core-shell particles

Abstract:

Tato prace se zabyva predikci reten.nich dat v rychlych gradientech pomoci izokratickych reten.nich model. na povrchov. poreznich kolonach s velikosti .astic 5 ?Ęm. K analyzam byly pou.ity standardy alkylbenzen. a fenolickych latek, ktere byly separovany na povrchov. poreznich kolonach s chemicky vazanymi stacionarnimi fazemi C18, fenyl-hexyl a bifenyl. Vybrane povrchov. porezni kolony byly pou.ity ve druhe dimenzi celostni dvoudimenzionalni kapalinove chromatografie k separaci fenolickych latek, zatimco k separaci v prvni dimenzi byla pou.ita kapilarni monoliticka zwitteriontova kolona.

Abstract:

This thesis deals with possibilities of prediction of gradient elution data using three different isocratic retention models on 5 ěm core-shell columns. Alkylbenzenes, phenolic acid a flavone standards were separated in fast gradient times between 1?10 min with different gradient range on three columns with different stationary phases (octadecyl, phenyl-hexyl and biphenyl). Furthermore, core-shell columns were employed in the second dimension of comprehensive two-dimensional liquid chromatography coupled with monolithic zwitterionic microcolumn in the first dimension.

Keywords

predikce retenčních dat, gradientová eluce, povrchově porézní částice, fenolické sloučeniny, dvoudimenzionální kapalinová chromatografie

Language used: Czech

Date on which the thesis was submitted / produced: 15. 5. 2015

Thesis defence

• Date of defence: 2. 6. 2015
• Supervisor: Ing. Tomáš Hájek, Ph.D.

University of Pardubice

Faculty of Chemical Technology

Master programme / field:
Chemistry and Technology of Foodstuffs / Evaluation and Analysis of Foodstuffs