Utilization of genome editing technology to knock out \kur{dnd1} gene in sturgeons – MSc. Trang VU THI
MSc. Trang VU THI
Master's thesis
Utilization of genome editing technology to knock out \kur{dnd1} gene in sturgeons
Utilization of genome editing technology to knock out \kur{dnd1} gene in sturgeons
Abstract:
In this study, for the first time we used CRISPR/Cas9 gene editing technology in sturgeons i.e., sterlets (Acipenser ruthenus). The sequences of sgRNA and primers were designed based on published dnd1 sterlet sequence. Each pair of sgRNA oligos after ligation ready duplex DNA fragment was cloned into vector pX330-U6-Chimeric_BB-CBh-hSpCas9 backbone and thereafter the transformation to competent cells …more
Language used: English
Date on which the thesis was submitted / produced: 24. 5. 2017
Thesis defence
- Supervisor: doc. Ing. Martin Pšenička, Ph.D.
Citation record
ISO 690-compliant citation record:
VU THI, Trang. \textit{Utilization of genome editing technology to knock out $\backslash$kur$\{$dnd1$\}$ gene in sturgeons}. Online. Master's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters. 2017. Available from: https://theses.cz/id/owo35q/.
The right form of listing the thesis as a source quoted
VU THI, Trang. Utilization of genome editing technology to knock out \kur{dnd1} gene in sturgeons. Č. Budějovice, 2017. diplomová práce (Ing.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Fakulta rybářství a ochrany vod
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Fakulta rybářství a ochrany vodUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of Fisheries and Protection of WatersMaster programme / field:
Agricultural Specialization / Fishery and Protection of Waters
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