Detection and isolation of relapsing fever spirochete, \kur{Borrelia miyamotoi}, from \kur{Ixodes ricinus} collected in the Czech Republic: separation of relapsing fever and Lyme disease spirochetes by cultivation on solid medium

Angela KIENBERGER

Bachelor's thesis

Detection and isolation of relapsing fever spirochete, \underline{Borrelia miyamotoi}, from \kur{Ixodes ricinus} collected in the Czech Republic: separation of relapsing fever and Lyme disease spirochetes by cultivation on solid medium

Abstract:

In this bachelor thesis, the focus of the study was on a newly discovered tick-born pathogen named \kur{Borrelia miyamotoi}, which belongs to the relapsing fever group and is transferred by the same \kur{Ixodes} species that transmit Lyme disease spirochetes. Very little is still known about the cultivation of \kur{B. miyamotoi} in-vitro, whereas it is of great importance since detailed characterization of the pathogen is necessary. Therefore, the interest of developing successful solid or liquid media for \kur{B. miyamotoi} in-vitro cultivation is steadily increasing. The aim of this thesis was to detect the presence of pathogenic for humans \kur{Borrelia miyamotoi} in hard ticks collected from different areas of the Czech Republic and to obtain a monoclonal population of \kur{B. miyamotoi}, while separating co-infected cultures on plates with solid medium.

Abstract:

In this bachelor thesis, the focus of the study was on a newly discovered tick-born pathogen named \kur{Borrelia miyamotoi}, which belongs to the relapsing fever group and is transferred by the same \kur{Ixodes} species that transmit Lyme disease spirochetes. Very little is still known about the cultivation of \kur{B. miyamotoi} in-vitro, whereas it is of great importance since detailed characterization of the pathogen is necessary. Therefore, the interest of developing successful solid or liquid media for \kur{B. miyamotoi} in-vitro cultivation is steadily increasing. The aim of this thesis was to detect the presence of pathogenic for humans \kur{Borrelia miyamotoi} in hard ticks collected from different areas of the Czech Republic and to obtain a monoclonal population of \kur{B. miyamotoi}, while separating co-infected cultures on plates with solid medium.

Keywords

\kur{Borrelia miyamotoi}, \kur{Borrelia burgdorferi} sensu lato, \kur{Ixodes ricinus}, Co-infections, Nested PCR, Reverse line blotting, monoclonal culture, mice-infection, MKP-F medium, plating, modified plating medium, molecular cloning

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 15. 12. 2022

Obhajoba závěrečné práce

• Vedúci: MSc. Maryna Holovčenko

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Bachelor programme / odbor:
Biological Chemistry / Biological Chemistry