IrAM4, částečná charakterizace molekuly podobné alpha2-makroglobulinu z klíštěte Ixodes ricinus

Markéta ABSOLONOVÁ

Bakalářská práce

IrAM4: Partial characterisation of a molecule similar to \recke{alpha}\dindex{2}-macroglobulin from a tick \kur{Ixodes ricinus}

Abstract:

Ixodes ricinus is a hard tick that can transmit several diseases that are capable of affecting humans. Among those are tick-borne encephalitis and Lyme disease. The focus of this work is on IrAM4, a member of tick ?2-macroglobulin family (?2M-F) of proteins which belong to the evolutionarily oldest constituents of the innate immune system. ?2-macroglobulins are protease inhibitors and act primarily in inactivation of proteases secreted by invading pathogens within their infection cycle. The aim of this study was to identify ?2-M of Ixodes ricinus (IrAM4) from corresponding ortholog of protein ?2-M named IsAM4 present in the genome of closely related Ixodes scapularis. The partial sequence was determined by amplification of cDNA and subsequent sequencing of PCR products. RT-PCR tissue profiling revealed that IrAM4 is present in ovaries and salivary glands but not in the tick gut. The recombinant fragment of IrAM4 was afterwards prepared for immunization of a rabbit and the obtained polyclonal antibodies were used for Western blot analysis. The results showed that IrAM4 is mainly present in the hemolymph and probably in salivary glands and ovaries but it is not expressed in the gut. The native IrAM4 seems to be composed of two disulfide bound subunits. However, the exact structure of the molecule was not analyzed in this work.

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 20. 5. 2011

Identifikátor: 19686

Obhajoba závěrečné práce

• Vedoucí: RNDr. Petr Kopáček, CSc.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Bakalářský studijní program / obor:
Biochemistry / Biological Chemistry