In vitro induction of polyploidy in Satureja montana L.

Jakub Vítek

Bakalářská práce

Abstract:

Satureja montana L. (winter savory) is a subshrub plant which belongs to family Lamiaceae. Its place of origin is in the Mediterranean region. It’s a widely known aromatic plant that finds use in traditional medicine, culinary and as a preservative in the food industry because of its high content of essential oils. Its aerial parts are used as traditional medicine for treatment of digestive issues. However, the plant is used for its bactericidal, antiviral, fungicidal etc. properties as well. S. montana contains aromatic substances with phenolic character in which the monoterpenes e.g. thymol, carvacrol and p-cymene are the most important. The aim of the research was to perform in vitro induced polyploidization of S. montana using antimitotic agent in order to obtain new genotypes with different ploidy level. Nodal segments of S. montana were used to induce in vitro polyploidization due to the increase the ploidy level of diploid plant (2n=30). Nodal segments were cultivated on MS (Murasnige and Skoog, 1962) medium free of PGR’s. Oryzalin, herbicide of dinitroaniline class, was used as an antimitotic agent for the in vitro induced somatic polyploidization. The experiment was performed on leafless nodal segments submerged in oryzalin solution in the concentration of 20 µM during 24 h and 48 h. Non treated plants were used as control. Cultivation conditions were 25 °C/day and 20 °C/night with light intensity of 3000 lx in photoperiod 16 h day, 8 h night. Treated plants were examined by flow cytometry to measure changes in ploidy level. In total, 6 new genotypes were obtained. Survival rate of affected plans was 6 % with polyploidy efficiency 100 %. After 4 weeks of observation, the following in vitro morphological parameters were evaluated: number of shoots, branching, number of nodal segments and roots. Obtained genotypes show morphological differences to control plants in mentioned parameters. Polyploidy induction of S. montana L. is possible in in vitro conditions and this method could be used in breeding programs.

Keywords

Satureja, In Vitro, Polyploidy

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 16. 4. 2021

Obhajoba závěrečné práce

• Vedoucí: prof. Ing. Eloy Fernández Cusimamani, Ph.D.

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Bakalářský studijní program / obor:
Zemědělská specializace / Zemědělství tropů a subtropů