Příprava monolitických kapilárních kolon s uhlíkovými mikroelektrodami

Ing. Martina Komendová

Master's thesis

Development of a monolithic capillary columns with carbon microelectrodes

Abstract:

Tato diplomova prace se zabyva p.ipravou monolitickych kapilarnich kolon s uhlikovymi mikroelektrodami. Pomoci uhlikovych a st.ibrnych mikrovlaken byla vytvo.ena pracovni a referentni elektroda, ktere byly nasledn. fixovany uvnit. k.emenne kapilary s vnit.nim pr.m.rem 530 ?Ęm. Vlastnosti p.ipraveneho elektrochemickeho detektoru byly optimalizovany z hlediska stability, linearity stanoveni a meze detekce. Mikroelektrodovy elektrochemicky detektor byl pou.it pro chromatografickou analyzu neurotransmiter..

Abstract:

The aim of this work is to prepare an integrated electrochemical detector inside fused-silica capillary with monolithic stationary phase. To reach this goal, we have used carbon and silver microfibers and used them as a working and reference electrode, respectively. After their integration inside the capillary, the preparation of presented electrochemical detector has been optimized in terms of stability, linearity and limits of detection. Finally, microelectrode capillary detector was used for chromatographic analysis of neurotransmitters.

Keywords

elektrochemická detekce, kapilární kapalinová chromatografie, monolitické stacionární fáze, neurotransmitery, uhlíková mikrovlákna

Language used: Czech

Date on which the thesis was submitted / produced: 20. 5. 2015

Thesis defence

• Date of defence: 1. 6. 2015
• Supervisor: RNDr. Jiří Urban, Ph.D.

University of Pardubice

Faculty of Chemical Technology

Master programme / field:
Chemistry / Analytical Chemistry