Zinc (ZnO) and titanium (TiO2) nanoparticles effect on micropropagation of Satureja Montana L.

Mohamed Noman

Bakalářská práce

Abstract:

Satureja montana L. commonly known as winter savory is an aromatic perennial herb used as a spice in food preparation and as a component in remedies in traditional medicine due to its essential oils. Several biological properties of its essential oils have been confirmed in recent studies such as antioxidant, anitmicrobal, and antiviral.Nano-scale particles with dimensions between 1 and 100 nm have been widely employed in different fields such as energy, transportation, cosmetics and agriculture due to their unique physico-chemical properties.This study deals with the effects of zinc oxide (ZnO) and titanium dioxide (TiO2) nanoparticles in different concentrations on the in vitro propagation of Satureja montana L.Nodal explants were cultivated on basic MS medium for 5 days and then supplemented with either zinc oxide (ZnO) or titanium dioxide (TiO2) nanoparticles in 20 mg/l, 40 mg/l and 60 mg/l. The nodal explants were cultivated for a 60-day period. The cultivation conditions were 16/8 day night cycle and a temperature of 25/23 °C day/night with a light intensity of 2500 lx. Measurements were taken every 10 days of number of sprouts, number of nods, sprout length, number of roots and root length.The results show that zinc oxide nanoparticles in 20 mg/l concentrations positively affected sprout length (55.5 mm -+ 39.6) however they slightly decreased the length of roots. In 40 mg/l ZnO nanoparticles inhibited root formation (3.21 +- 4.29) and length (10.64 mm +- 5.81), while 60 mg ZnO didnt have any effect on growth of nodal explants. TiO2 nanoparticles exhibited toxic effects on root length growth.

Keywords

Lamiaceae, In Vitro, Micropropagation, Nanoparticles

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 2. 5. 2017

Obhajoba závěrečné práce

• Vedoucí: doc. Dr. Ing. Eloy Fernández Cusimamani

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Bakalářský studijní program / obor:
Zemědělská specializace / Zemědělství tropů a subtropů