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Bc. Bruno Petrus

Bachelor's thesis

Segmentation of Membrane-Stained Cells in Image Data of Organoids

Segmentation of Membrane-Stained Cells in Image Data of Organoids
Abstract:
V tejto práci je prezentovaný dvojkrokový proces spracovania obrazu, ktorý má za cieľ segmentovať obrazové dáta organoidov. Tieto dáta obsahujú dva obrazové kanály, v jednom sú ofarbené membrány buniek a v druhom jadrá buniek. V prvej fáze sa vytvorí hrubá segmentácia jednotlivých buniek pomocou analýzy Laplaceovho operátora a watershed algoritmu v kanáli s ofarbenými membránami. V druhej fáze je segmentácia …more
Abstract:
In this thesis, we present a two-stage image analysis pipeline for segmenting membrane-stained and nuclei-stained image data of organoids captured using a fluorescence microscope. In the first stage, a coarse segmentation of individual cells in the membrane channel is obtained by analysing the Laplacian operator's response and watershed-driven tesselation. In the second stage, the coarse segmentation …more
 

Keywords

segmentation image processing scikit-image watershed thresholding fluorescence microscopy zero-crossing
 
Language used: English
Date on which the thesis was submitted / produced: 18. 5. 2023
Identifier: https://is.muni.cz/th/igph2/

Thesis defence

  • Date of defence: 27. 6. 2023
  • Supervisor: doc. RNDr. Martin Maška, Ph.D.
  • Reader: doc. RNDr. Pavel Matula, Ph.D.

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Institution archiving the thesis and making it accessible: Masarykova univerzita, Fakulta informatiky
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Masaryk University

Faculty of Informatics

Bachelor programme / field:
Informatics / Informatics

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