Characterizing the Impact of the SARS-CoV-2 British Mutation on Mouse Lung Tissue Using 3D SBF- SEM Imaging

Ana- Maria COROIANU

Bachelor's thesis

3D SBF-SEM analysis of effect that SARS-COV 2 British mutation has on mouse lungs

Abstract:

This thesis investigates ultrastructural changes in mouse lungs after infection of the severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of the global COVID-19 pandemic. Focusing on the British SARS- CoV-2 B.1.1.7 variant, the research aims to identify and describe what is happening in the infected lung tissue during the early infection stages. By analyzing serial block face scanning electron microscopy (SBF-SEM) datasets at three time points, the thesis aims to contribute insights into the onset and the progression of the disease.

Abstract:

This thesis investigates ultrastructural changes in mouse lungs after infection of the severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of the global COVID-19 pandemic. Focusing on the British SARS- CoV-2 B.1.1.7 variant, the research aims to identify and describe what is happening in the infected lung tissue during the early infection stages. By analyzing serial block face scanning electron microscopy (SBF-SEM) datasets at three time points, the thesis aims to contribute insights into the onset and the progression of the disease.

Keywords

CoV-2, COVID-19 pandemic, Coronavirus-2, Electron Microscopy, SBF-SEM, Serial block face scanning electron microscopy, mouse lung infection.

Language used: English

Date on which the thesis was submitted / produced: 3. 5. 2024

Thesis defence

• Supervisor: RNDr. Jiří Týč, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Bachelor programme / field:
Biological Chemistry / Biological Chemistry