Mgr. Jitka Ždánská

Bachelor's thesis

Optimalizace produkce cukr-vázajících proteinů z Aspergillus fumigatus v hostitelském organismu Escherichia coli

Optimization of production of sugar-binding proteins from Aspergillus fumigatus in Escherichia coli host organism
Abstract:
V této bakalářské práci je zkoumána produkce lektinu AFL2 z plísně Aspergillus fumigatus. Jedná se o hypotetický protein, který zatím nebyl izolován v rozpustné formě. Cílem bakalářské práce bylo optimalizovat expresi tohoto proteinu v hostitelském organismu E. coli. Byla optimalizována teplota exprese proteinu, koncentrace induktoru exprese i bod indukce. Bylo testováno několik kmenů E. coli a vliv …more
Abstract:
In this bachelor thesis I deal with production of lectin AFL2 from Aspergillus fumigatus. It is hypothetical protein that was not yet isolated in soluble form. The goal of this bachelor thesis was the optimization of protein expression in Escherichia coli host organism. There were many optimization: the temperature of protein expression, the concentration of protein inducer and the point of induction …more
 
 
Language used: Czech
Date on which the thesis was submitted / produced: 15. 5. 2013

Thesis defence

  • Date of defence: 17. 6. 2013
  • Supervisor: prof. RNDr. Michaela Wimmerová, Ph.D.

Citation record

Full text of thesis

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Institution archiving the thesis and making it accessible: Masarykova univerzita, Přírodovědecká fakulta