Značení Babesia divergens kalcium-dependentní proteinkinázy 5 (BdCDPK5) metodou knock-in

Tereza ŽIROVNICKÁ

Bakalářská práce

Abstract:

Babesia divergens is a tick-borne parasite that infects both bovines and humans, causing babesiosis. Despite its increasing impact, there are considerable limitations in understandng the key life cycle strategies of this parasite and the molecules orchestrating them. One of the crucial signaling molecules is the class of Calcium Dependent Protein Kinases (CDPKs), expected to be involved in regulating the B. divergens complex life cycle in both vertebrate and tick hosts. This thesis aims to create transgenic parasites with targeted BdCDPK5 signaling molecule using knock-in technology. In this study, we designed and successfully assembled a plasmid for knock-in targeting BdCDPK5 fused with selected reporter genes. We transfected the plasmid into B. divergens in vitro culture using nucleofection to generate transgenic parasites, with WR99210 used as a selective drug. The resulting transgenic line will serve for a detailed characterization of BdCDPK5 function throughout the B. divergens life cycle and the localization of this enzyme in the parasite cell using various microscopy techniques.

Klíčová slova

Babesia divergens, CDPKs, genové manipulace, knock-in, BdCDPK5

Jazyk práce: čeština

Datum vytvoření / odevzdání či podání práce: 12. 4. 2024

Obhajoba závěrečné práce

• Vedoucí: RNDr. Marie Jalovecká, Ph.D.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Bakalářský studijní program / obor:
Biologie / Biologie