Mgr. Helena Peschelová, Ph.D.

Doctoral thesis

The use of CRISPR/Cas9 for investigating targeted therapy opportunities in B-cell chronic lymphocytic leukemia

The use of CRISPR/Cas9 for investigating targeted therapy opportunities in B-cell chronic lymphocytic leukemia
Abstract:
Chronická lymfocytární leukemie (CLL) je nejčastější leukemií dospělých a její výzkum je komplikován nedostatkem reprezentativních experimentálních modelů. Tato práce se zabývá tvorbou a charakterizací nových isogenních modelů CLL pomocí technologie CRISPR/Cas9. Byly připraveny buněčné linie nesoucí knockout nebo knock-in vybrané pro relevantní mutace (ATM, TP53, SF3B1, NOTCH1, MYD88, MS4A1) a ověřeny …more
Abstract:
Chronic lymphocytic leukemia (CLL) is the most common leukemia in adults, yet its research is limited by the scarcity of reliable experimental models. This thesis focuses on the development and characterization of novel isogenic CLL models using CRISPR/Cas9 technology. Cell lines carrying knockout or knock-in mutations in relevant genes (ATM, TP53, SF3B1, NOTCH1, MYD88, MS4A1) were established and …more
 

Keywords

chronická lymfocytární leukemie CRISPR/Cas9 CRISPR screening buněčné modely syntetická letalita CAR T-lymfocyty chronic lymphocytic leukemia cell models synthetic lethality CAR T lymphocytes
 
Language used: English
Date on which the thesis was submitted / produced: 12. 9. 2025
Identifier: https://is.muni.cz/th/mb2zx/

Thesis defence

  • Date of defence: 1. 12. 2025
  • Supervisor: Mgr. Michal Šmída, Dr. rer. nat.
  • Reader: RNDr. Matouš Hrdinka, Ph.D., Ph.D. Firczuk Malgorzata

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